GSM1669153: JC2585_Xenograft_MCF7_PR_E2+PG r8; Homo sapiens; ChIP-Seq - SRA

SRX1012758: GSM1669153: JC2585_Xenograft_MCF7_PR_E2+PG r8; Homo sapiens; ChIP-Seq
3 ILLUMINA (Illumina HiSeq 2000) runs: 19.6M spots, 979.5M bases, 613.8Mb downloads

Submitted by: Gene Expression Omnibus (GEO)

Study: ChIP-seq of ER and PR in xenografted MCF7 tumors grown in mice [xenograft]

PRJNA282506 • SRP057757 • All experiments • All runs

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Exploring effect of estrogen and/or progesterone treatment on xenografts tumors in vivo Overall design: MCF7 cells implanted in mice (two each), subjected to different treatment. Six replicates of each condition we ChIPed.

Sample: JC2585_Xenograft_MCF7_PR_E2+PG r8

SAMN03570732 • SRS924538 • All experiments • All runs

Organism: Homo sapiens

Library:

Instrument: Illumina HiSeq 2000

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 4 sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendationas described in Chandra, T. et al. Independence of Repressive Histone Marks and ChromatinCompaction during Senescent Heterochromatic Layer Formation. Mol Cell 47, 203–214 (2012)

Experiment attributes:

GEO Accession: GSM1669153

Links:

External link: GEO Sample GSM1669153

NCBI link: NCBI Entrez (gds)

Runs: 3 runs, 19.6M spots, 979.5M bases, 613.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR2000989	5,200,460	260M	165.2Mb	2015-05-22
SRR2000990	7,078,666	353.9M	222.3Mb	2015-05-22
SRR2000991	7,309,973	365.5M	226.3Mb	2015-05-22

ID:: 1467610

SRA

Sequence Read Archive

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